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Journal: International Journal of Molecular Sciences
Article Title: Reactive Oxygen Species Mediate 6c-Induced Mitochondrial and Lysosomal Dysfunction, Autophagic Cell Death, and DNA Damage in Hepatocellular Carcinoma
doi: 10.3390/ijms222010987
Figure Lengend Snippet: Compound 6c induced DNA damage in HepG2 cells. ( A ) Shape changes of nucleus after 6c (20 μM) treatment for 24 h in HepG2 cells. 6c-treated cells were stained with Hoechst 33342, and the images were captured using confocal microscopy. ( B ) The foci of γH2A.X in HepG2 cells treated with or without 6c (20 μM) for 24 h were detected using confocal microscopy. ( C ) Representative images of alkaline comet assay. HepG2 cells were treated with or without 6c (20 μM) for 24 h. ( D ) The expression of proteins related with DNA damage and p53 pathway after 6c treatment for 24 h was determined using Western blot. ( E ) Effects of p53 inhibitor PFTα on 6c-induced cell viability inhibition (up) and LC3-II expression (down). HepG2 cells were pretreated with PFTα (10 μM) for 1 h followed by treatment with 6c (20 μM) for 24 h. Cell viability was tested using MTT assays, and the expression of LC3 was detected using Western blot. ( F ) The expression of LC3, p53, and γH2A.X was determined using Western blot. HepG2 cells were pretreated with 3-MA for 1 h followed by treatment with 6c (20 μM) for 24 h.
Article Snippet: The measurement of DNA damage was performed using the
Techniques: Staining, Confocal Microscopy, Alkaline Single Cell Gel Electrophoresis, Expressing, Western Blot, Inhibition